HDAC2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q92769 |
| Gene Id | 3066 |
| Gene Symbols | HDAC2 |
| Protein Name / Synonyms | HDAC2, HD2, RPD3, YAF1, histone deacetylase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
51kDa/55kDa
Observed MW
60kDa
Immunogen
A synthetic peptide of human HDAC2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HDAC2 antibody (144-02084) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody (144-02084) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC2 antibody (144-02084). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug HDAC2 antibody (144-02084). Western blot was performed from the immunoprecipitate using HDAC2 antibody (144-02084) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.