Alkaline Phosphatase (Tissue Non-Specific) antibody, 0.48 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 249 |
| Protein Name / Synonyms | Alkaline Phosphatase (Tissue Non-Specific) |
| Concentration (lot specific) | 0.48 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AP-TNAP antibody, APTNAP antibody, FLJ40094 antibody, FLJ93059 antibody, HOPS antibody, MGC161443 antibody, MGC167935 antibody, TNAP antibody, TNSALP antibody, ALPL antibody, alkaline phosphomonoesterase antibody, liver/bone/kidney-type alkaline phosphatase antibody, "alkaline phosphatase, tissue-nonspecific isozyme antibody", alkaline phosphatase liver/bone/kidney isozyme antibody, tissue-nonspecific ALP antibody, glycerophosphatase antibody, "alkaline phosphatase, liver/bone/kidney antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
57 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 359 (P05186) of Alkaline Phosphatase (Tissue Non-Specific)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using alkaline phosphatase (liver/bone/kidney)(107-10239) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-10239 diluted at 1:1000
A: Hela
7.5% SDS PAGE
107-10239 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.