Rabbit Anti-Human ATP5C1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P36542 |
| Gene Id | 509 |
| Gene Symbols | ATP5C1 |
| Protein Name / Synonyms | ATP5C1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ATP5C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-67 amino acids from the N-terminal region of human ATP5C1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ATP5C1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Monkey
Other Names
ATP5C1; ATP5C; ATP5CL1; ATP synthase subunit gamma, mitochondrial; F-ATPase gamma subunit
NCBI Accession #
NP_001001973.1;NP_005165.1
Other Accession #
P35435; Q91VR2; Q4R5B0; P05631
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
32996
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of ATP5C1 Antibody (N-term) (Cat# 102-18201) in HL-60 cell line lysates (35ug/lane).ATP5C1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ATP5C1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | ATP5C1 Antibody (N-term) (Cat# 102-18201) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.