BCL2L15 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 440603 |
| Protein Name / Synonyms | BCL2L15 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
Bfk antibody, C1orf178 antibody, FLJ22588 antibody, BCL2L15 antibody, pro-apoptotic Bcl-2 protein antibody, bcl-2-like protein 15 antibody, bcl2-L-15 antibody, BCL2-like 15 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
18 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 163 of BCL2L15 (Uniprot ID# Q5TBC7)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
BCL2L15 antibody detects BCL2L15 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCL2L15 protein stained by BCL2L15 antibody (107-10941) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCL2L15 protein stained by BCL2L15 antibody (107-10941) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using BCL2L15(107-10941) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
107-10941 diluted at 1:1000
A: Raji
12% SDS PAGE
107-10941 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.