Rabbit Anti-Human BDNF (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P23560 |
| Gene Id | 627 |
| Gene Symbols | BDNF |
| Protein Name / Synonyms | BDNF (C-term) |
| Clonality | Polyclonal |
| Preparation | This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
BDNF is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this protein is reduced in both Alzheimer's and Huntington disease patients. This protein may play a role in the regulation of stress response and in the biology of mood disorders.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Pig
Other Names
BDNF; Brain-derived neurotrophic factor; Abrineurin
NCBI Accession #
NP_001137277.1;NP_001137278.1;NP_001137279.1;NP_001137280.1;NP_001137281.1;NP_001137282.1;NP_001137283.1;NP_001137284.1;NP_001137285.1;NP_001137286.1;NP_001137288.1;NP_001700.2;NP_733927.1;NP_733928.1
Other Accession #
P23363; P14082; P21237; Q95106
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
27818
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of BDNF Antibody (C-term)(Cat# 102-17740) in CEM cell line lysates (35ug/lane). BDNF (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain reacted with BDNF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of BDNF Antibody (C-term)(Cat# 102-17740) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | Flow cytometric analysis of CEM cells using BDNF Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.