Rabbit Anti-Human CD4 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P01730 |
| Gene Id | 920 |
| Gene Symbols | CD4 |
| Protein Name / Synonyms | CD4 (N-term) |
| Clonality | Polyclonal |
| Preparation | This CD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the N-terminal region of human CD4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Research Area | Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Specificity
Other Names
CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD_antigen=CD4; Flags: Precursor
NCBI Accession #
NP_000607.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
51111
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
 | Western blot analysis of CD4 Antibody (N-term) in CEM cell line lysates (35ug/lane). CD4(arrow) was detected using the purified Pab. |
 | Western blot analysis of CD4 (arrow) using rabbit polyclonal CD4 Antibody (N-term) (RB12862). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies). |
 | CD4 Antibody (N-term) (Cat# 102-12749) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.