Rabbit Anti-Human CTNNB1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P35222 |
| Gene Id | 1499 |
| Gene Symbols | CTNNB1 |
| Protein Name / Synonyms | CTNNB1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This CTNNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-106 amino acids from the N-terminal region of human CTNNB1. |
| Concentration (lot specific) | 0.400 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Wnt-beta-Catenin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Xenopus
Other Names
CTNNB1; CTNNB; Catenin beta-1; Beta-catenin
NCBI Accession #
NP_001091679.1;NP_001091680.1;NP_001895.1
Other Accession #
P26233; Q9WU82; Q02248; Q0VCX4; NP_001091679.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
85497
Recommended Dilutions
WB: 1:2000; IHC: 1:50; FC: 1:50; IF: 1:50
 | CTNNB1 Antibody (N-term) (Cat# 102-18482) western blot analysis in 293,T47D cell line lysates (35ug/lane).This demonstrates the CTNNB1 antibody detected the CTNNB1 protein (arrow). |
 | CTNNB1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CTNNB1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | CTNNB1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of CTNNB1 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). |
 | Confocal immunofluorescent analysis of CTNNB1 Antibody (N-term) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.