Rabbit Anti-Human FADS2 (N-term) Antibody, 200 µl
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Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O95864 |
| Gene Id | 9415 |
| Gene Symbols | FADS2 |
| Protein Name / Synonyms | FADS2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human FADS2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.
Specificity
Predicted Reactivity
Monkey
Other Names
FADS2; Fatty acid desaturase 2; Delta(6) fatty acid desaturase
NCBI Accession #
NP_001268430.1;NP_001268431.1;NP_004256.1
Other Accession #
Q4R749; NP_004256.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
52259
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Fluorescent image of A549 cell stained with FADS2 Antibody (N-term)(Cat# 102-11488/SA110322AN).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly. |
 | FADS2 Antibody (N-term) (Cat# 102-11488) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow). |
 | FADS2 Antibody (N-term) (Cat# 102-11488)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FADS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | FADS2 Antibody (N-term) (Cat# 102-11488) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.