GAPDH antibody, 0.69 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 2597 |
| Protein Name / Synonyms | GAPDH |
| Concentration (lot specific) | 0.69 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
G3PD antibody, GAPD antibody, MGC88685 antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
36 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 335 of GAPDH (Uniprot ID# P04406)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of Salivary gland cancer, using GAPDH(107-10183) antibody at 1:500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH(107-10183) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
GAPDH antibody detects GAPDH protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg mouse brain lysate/extract
D. 30 µg PC-12 whole cell lysate/extract
E. 30 µg rat brain lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg mouse brain lysate/extract
D. 30 µg PC-12 whole cell lysate/extract
E. 30 µg rat brain lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
GAPDH antibody detects GAPDH protein by Western blot analysis.
A. 0.1 µg 293T whole cell lysate/extract
B. 1 µg 293T whole cell lysate/extract
C. 10 µg 293T whole cell lysate/extract
12 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
A. 0.1 µg 293T whole cell lysate/extract
B. 1 µg 293T whole cell lysate/extract
C. 10 µg 293T whole cell lysate/extract
12 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
GAPDH antibody detects GAPDH protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract |
D. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract |
D. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
GAPDH antibody detects GAPDH protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw 264.7 whole cell lysate/extract
G. 30 µg C2Cl2 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw 264.7 whole cell lysate/extract
G. 30 µg C2Cl2 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
GAPDH antibody detects GAPDH protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
GAPDH antibody detects GAPDH protein by western blot analysis.
A. 20 µg 293T whole cell lysate/extract
B. 10 µg 293T whole cell lysate/extract
C. 5 µg 293T whole cell lysate/extract
D. 1 µg 293T whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
A. 20 µg 293T whole cell lysate/extract
B. 10 µg 293T whole cell lysate/extract
C. 5 µg 293T whole cell lysate/extract
D. 1 µg 293T whole cell lysate/extract
10 % SDS-PAGE
GAPDH antibody (107-10183) dilution: 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.