Rabbit Anti-Human GTF2I (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P78347 |
| Gene Id | 2969 |
| Gene Symbols | GTF2I |
| Protein Name / Synonyms | GTF2I (N-term) |
| Clonality | Polyclonal |
| Preparation | This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the N-terminal region of human GTF2I. |
| Concentration (lot specific) | 0.400 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 7, 13 and 21.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
GTF2I; BAP135; WBSCR6; General transcription factor II-I; Bruton tyrosine kinase-associated protein 135; SRF-Phox1-interacting protein; Williams-Beuren syndrome chromosomal region 6 protein
NCBI Accession #
NP_001157108.1;NP_001267729.1;NP_001509.3;NP_127492.1;NP_127493.1;NP_127494.1
Other Accession #
Q5U2Y1; Q9ESZ8; NP_001157108.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
112416
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of Hela cell stained with GTF2I Antibody (N-term)(Cat# 102-13682/SA111012BI).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). GTF2I immunoreactivity is localized to Nucleus significantly. |
 | GTF2I Antibody (N-term) (Cat# 102-13682) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.