HLA-DMB antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3109 |
| Protein Name / Synonyms | HLA-DMB, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
D6S221E antibody, RING7 antibody, HLA-DMB antibody, really interesting new gene 7 protein antibody, MHC class II HLA-DMB antibody, "HLA class II histocompatibility antigen, DM beta chain antibody", "class II histocompatibility antigen, M beta chain antibody", MHC class II antigen HLA-DM beta chain antibody, MHC class II antigen DMB antibody, "major histocompatibility complex, class II, DM beta antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 199 and 263 of HLA-DMB (Uniprot ID# P28068)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Colon ca, using HLA-DMB(107-10771) antibody at 1:250 dilution.
Western Blot analysis of HLA-DMB expression in transfected 293T cell line by HLA-DMB polyclonal antibody.
A:Non-transfected lysate.
B:HLA-DMB transfected lysate.
12% SDS PAGE
107-10771 diluted at 1:500
A:Non-transfected lysate.
B:HLA-DMB transfected lysate.
12% SDS PAGE
107-10771 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.