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Antigen Information
Protein Name & Synonyms:
HRK
Target Species:
- Human
Antibody Specifications
Applications:
Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Expected cross reactivity from sequence similarity: mouse and rat
This product detects a band of approximately 13kDa in mouse pancreas tissue lysate.
Specificity
This product detects Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and hematopoietic cell apoptosis, in particular to growth factor deprivation.
Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight, HRK often migrates at 12-15kD.
Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight, HRK often migrates at 12-15kD.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
15 amino acid peptide from near the center of human HRK (NP_003797).
Preservative:
| 0.02% | Sodium Azide |
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
