Rabbit Anti-Human KLF4 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43474 |
| Gene Id | 9314 |
| Gene Symbols | KLF4 |
| Protein Name / Synonyms | KLF4 |
| Clonality | Polyclonal |
| Preparation | This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-504 amino acids from human KLF4. |
| Concentration (lot specific) | 0.420 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Kr
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
KLF4; EZF; GKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
NCBI Accession #
NP_004226.3
Other Accession #
Q60793; Q9ET58; Q60843; Q9Y5W3
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
54671
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Immunofluorescent analysis of U251 cells, using KLF4 Antibody (Cat# 102-15099). 102-15099 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red). |
 | Western blot analysis of KLF4-S457 (Cat# 102-15099) in MDA-MB231, NCI-H460 cell line lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab. |
 | Fluorescent confocal image of HeLa cells stained with 102-15099 KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-15099 KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm. |
 | Fluorescent confocal image of SY5Y cells stained with 102-15099 KLF4 (S457) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-15099 KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.