LOXL2 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 4017 |
| Protein Name / Synonyms | LOXL2 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
LOR2 antibody, WS9-14 antibody, LOXL2 antibody, lysyl oxidase-related protein 2 antibody, lysyl oxidase homolog 2 antibody, lysyl oxidase-like protein 2 antibody, lysyl oxidase-related protein WS9-14 antibody, lysyl oxidase related 2 antibody, lysyl oxidase-like 2 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
87 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 454 and 742 of LOXL2 (Uniprot ID# Q9Y4K0)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
107-10056 diluted at 1:1000
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
107-10056 diluted at 1:1000
LOXL2 antibody detects LOXL2 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
LOXL2 antibody (107-10056) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
LOXL2 antibody (107-10056) dilution: 1:1000
LOXL2 antibody detects LOXL2 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
LOXL2 antibody (107-10056) dilution: 1:500.
Sample: Paraffin-embedded human ovarian carcinoma.
LOXL2 antibody (107-10056) dilution: 1:500.
LOXL2 antibody validation by shRNA knock-down.
Upperpanel: LOXL2 antibody (107-10056)
Lower panel: beta Actin antibody
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg whole cell lysate/extract of LOXL2 shRNA-transfected HeLa cells
C. 30 µg whole cell lysate/extract of LOXL2 shRNA-transfected HeLa cells
7.5 % SDS-PAGE
LOXL2 antibody (107-10056) dilution: 1:500
beta Actin antibody dilution: 1:20000
Upperpanel: LOXL2 antibody (107-10056)
Lower panel: beta Actin antibody
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg whole cell lysate/extract of LOXL2 shRNA-transfected HeLa cells
C. 30 µg whole cell lysate/extract of LOXL2 shRNA-transfected HeLa cells
7.5 % SDS-PAGE
LOXL2 antibody (107-10056) dilution: 1:500
beta Actin antibody dilution: 1:20000
LOXL2 antibody detects LOXL2 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
LOXL2 antibody (107-10056) dilution: 1:1000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
LOXL2 antibody (107-10056) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.