Rabbit Anti-Human NFYB (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P25208 |
| Gene Id | 4801 |
| Gene Symbols | NFYB |
| Protein Name / Synonyms | NFYB (N-term) |
| Clonality | Polyclonal |
| Preparation | This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB. |
| Concentration (lot specific) | 0.420 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Chicken
Other Names
NFYB; HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B
NCBI Accession #
NP_006157.1
Other Accession #
P63140; P63139; P25207; Q32KW0; NP_006157.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
22831
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | NFYB Antibody (N-term) (Cat# 102-12436) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term)(Cat# 102-12436). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.