NT5C2 antibody [N2C1], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 22978 |
| Protein Name / Synonyms | NT5C2, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
GMP antibody, NT5B antibody, PNT5 antibody, cN-II antibody, NT5C2 antibody, cytosolic 5'-nucleotidase II antibody, "5'-nucleotidase (purine), cytosolic type B antibody", IMP-specific 5'-NT antibody, purine 5' nucleotidase antibody, cytosolic purine 5'-nucleotidase antibody, "5'-nucleotidase, cytosolic II antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
65 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 108 and 362 of NT5C2 (Uniprot ID# P49902)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
107-10554 diluted at 1:500
A: mouse liver
7.5% SDS PAGE
107-10554 diluted at 1:500
Immunohistochemical analysis of paraffin-embedded OVCA, using NT5C2(107-10554) antibody at 1:100 dilution.
Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
7.5% SDS PAGE
107-10554 diluted at 1:1000
A:H1299
B:HeLa S3
7.5% SDS PAGE
107-10554 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.