PDI antibody [N1N3], 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5034 |
| Protein Name / Synonyms | PDI |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DSI antibody, ERBA2L antibody, GIT antibody, P4Hbeta antibody, PDI antibody, PDIA1 antibody, PHDB antibody, PO4DB antibody, PO4HB antibody, PROHB antibody, P4HB antibody, cellular thyroid hormone-binding protein antibody, protein disulfide-isomerase antibody, "protein disulfide isomerase family A, member 1 antibody", glutathione-insulin transhydrogenase antibody, protein disulfide isomerase/oxidoreductase antibody, "procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide antibody", collagen prolyl 4-hydroxylase beta antibody, protocollagen hydroxylase antibody, prolyl 4-hydroxylase subunit beta antibody, thyroid hormone-binding protein p55 antibody, protein disulfide isomerase-associated 1 antibody, p55 antibody, "prolyl 4-hydroxylase, beta polypeptide antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
57 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 23 and 227 of PDI (Uniprot ID# P07237)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using PDI(107-10262) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using PDI(107-10262) antibody at 1:100 dilution.
PDI antibody [N1N3] detects PDI protein at cytosol and membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PDI antibody [N1N3] (107-10262) dilution: 1:500.
Sample: Paraffin-embedded mouse colon.
PDI antibody [N1N3] (107-10262) dilution: 1:500.
Sample (20 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
107-10262 diluted at 1:10000
A: mouse liver
7.5% SDS PAGE
107-10262 diluted at 1:10000
Sample(30 µg of whole cell lysate)
A:293T
B:A431
C:H1299
7.5% SDS PAGE
107-10262 diluted at 1:500
A:293T
B:A431
C:H1299
7.5% SDS PAGE
107-10262 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.