Rabbit Anti-Human Phospho-EGFR(Y1016) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P00533 |
| Gene Id | 1956 |
| Gene Symbols | EGFR |
| Protein Name / Synonyms | Phospho-EGFR(Y1016) |
| Clonality | Polyclonal |
| Preparation | This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1016 of human EGFR. |
| Concentration (lot specific) | 0.300 mg/ml |
| Recommended Applications | Dot Blotting, Western Blotting |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. It is involved in the control of cell growth and differentiation.
Specificity
Predicted Reactivity
Mouse
Other Names
EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
NCBI Accession #
NP_005219.2;NP_958439.1;NP_958440.1;NP_958441.1
Other Accession #
Q01279
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
134277
Recommended Dilutions
WB: 1:8000; DB: 1:500
 | Western blot analysis of lysate from A431 cells(from left to right),untreated or treated with EGF at 100ng/ml,using Phospho-EGFR-pY1016 Antibody (Cat# 102-15455) or EGFR-pS695 Antibody.Lysate at 15ug per lane.102-15455 was diluted at 1:8000 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. |
 | Dot blot analysis of anti-EGFR B1 Phospho-specific Pab (Cat# 102-15455) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.