Rabbit Anti-Human Phospho-LIN28(S134) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9H9Z2 |
| Gene Id | 79727 |
| Gene Symbols | LIN28A |
| Protein Name / Synonyms | Phospho-LIN28(S134) |
| Clonality | Polyclonal |
| Preparation | This LIN28 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human LIN28. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, Dot Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
LIN28 is probable transcription factor. It plays a critical role in the control of immune response.
Specificity
Other Names
LIN28A; CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1
NCBI Accession #
NP_078950.1
Other Accession #
NP_078950
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
22743
Recommended Dilutions
IF: 1:100; DB: 1:500
 | Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15537 phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914). |
 | Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab (Cat# 102-15537) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.