Rabbit Anti-Human Prohibitin (PHB1) (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P35232 |
| Gene Id | 5245 |
| Gene Symbols | PHB |
| Protein Name / Synonyms | Prohibitin (PHB1) (N-term) |
| Clonality | Polyclonal |
| Preparation | This Prohibitin (PHB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human Prohibitin (PHB1). |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | FC, Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.
Specificity
Predicted Reactivity
Bovine, Chicken
Other Names
PHB; Prohibitin
NCBI Accession #
NP_001268425.1;NP_001268644.1;NP_002625.1
Other Accession #
P84173; Q3T165
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
29804
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | PHB1 Antibody (Human N-term) (Cat# 102-15074) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PHB1 antibody detected the PHB1 protein (arrow). |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma with Prohibitin (PHB1) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.