Proteasome 20S alpha 3 antibody, 0.36 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5684 |
| Protein Name / Synonyms | Proteasome 20S alpha 3 |
| Concentration (lot specific) | 0.36 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HC8 antibody, MGC12306 antibody, MGC32631 antibody, PSC3 antibody, PSMA3 antibody, proteasome subunit alpha type-3 antibody, proteasome subunit C8 antibody, multicatalytic endopeptidase complex subunit C8 antibody, proteasome component C8 antibody, macropain subunit C8 antibody, proteasome (prosome, macropain) subunit, alpha type, 3 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
28 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 137 (P25788) of Proteasome 20S alpha 3
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Proteasome 20S alpha 3 antibody detects PSMA3 protein by Western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg JC whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 3 antibody (107-11072) dilution: 1:1000
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg JC whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 3 antibody (107-11072) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded U87 xenograft , using 20S Proteasome alpha3 (107-11072) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
107-11072 diluted at 1:1000
A: Molt-4
12% SDS PAGE
107-11072 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.