Protein Name & Synonyms:
PUMA alpha
Applications:
Immunohistology - Paraffin, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml
Preparation:
Antisera to human Bcl-2 binding component 3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Species Reactivity:
Human
This product detects a band of approximately 23kDa in K562 cell lysates.
This product recognizes the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3. Bcl-2 binding component 3 is a pro-apoptotic member of the BCL-2 family.
Bcl-2 binding component 3 is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Antisera to human Bcl-2 binding component 3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3.
Expiration:
12 months from the date of shipment when stored properly.