Rad9 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 5883 |
| Protein Name / Synonyms | Rad9 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
RAD9 antibody, RAD9A antibody, DNA repair exonuclease rad9 homolog A antibody, cell cycle checkpoint control protein RAD9A antibody, hRAD9 antibody, RAD9 homolog A (S. pombe) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
43 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 197 and 341 of Rad9
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Rad9 antibody (107-10178) dilution: 1:500.
Sample: Paraffin-embedded HBL435 xenograft.
Rad9 antibody (107-10178) dilution: 1:500.
Rad9 antibody detects RAD9A protein by Western blot analysis.
A. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (107-10178) dilution: 1:500
A. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (107-10178) dilution: 1:500
Rad9 antibody detects RAD9A protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (107-10178) dilution: 1:1000
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (107-10178) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 (107-10178) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: Hela
10% SDS PAGE
107-10178 diluted at 1:1000
A: A431
B: Hela
10% SDS PAGE
107-10178 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.