Synonyms
FLJ10377 antibody, RBM28 antibody, 2810480G15Rik antibody, RNA-binding protein 28 antibody, RNA-binding motif protein 28 antibody, RNA binding motif protein 28 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
86 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 475 and 745 of RBM28 (Uniprot ID# Q9NW13)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using RBM28(107-10909) antibody (Green) at 1:100 dilution. Alpha-tubulin filaments were labeled at 1:2500.

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
107-10909 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.