ELISA (recommended work dilution= 1:2,000-5,000), Western Blotting( recommended work dilution= 2-5 µg/ml)
Immunogen was peptide derived from human RELM beta. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction of immunized serum was purified by Protein G/A affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
RELM beta belongs to the family of tissue specific cytokine-rich secretory proteins. There is high homology among the three known members of the RELM-FIZZ family, Resistin, RELM alpha and RELM beta.They are all 85-94 amino acid secreted proteins with a highly conserved C-terminal domain characterized by 10 cysteine residues with unique spacing motif. Studies on RELM-FIZZ Family have shown that it may play important role in obesity. RELM beta is expressed in the epithelium of the colon and small bowel.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The rabbit anti-human RELM beta antibody detected specifically human recombinant protein and cellular protein from cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
- He, W., M. L. Wang, H. Q. Jiang, C. M. Steppan, M. E. Shin, M. C. Thurnheer, J. J. Cebra, M. A. Lazar, G. D. Wu. 2003. Bacterial colonization leads to the colonic secretion of RELMbeta/FIZZ2, a novel goblet cell-specific protein. Gastroenterology 125: 1388-1397.
- Artis, D., M. L. Wang, S. A. Keilbaugh, W. He, M. Brenes, G. P. Swain, P. A. Knight, D. D. Donaldson, M. A. Lazar, H. R. Miller, et al 2004. RELMbeta/FIZZ2 is a goblet cell-specific immuneeffector molecule in the gastrointestinal tract. Proc. Natl. Acad. Sci. USA 101: 13596-13600.
12 months from the date of shipment when stored properly.