Ribosome binding protein 1 antibody [C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 6238 |
| Protein Name / Synonyms | Ribosome binding protein 1, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp586A1420 antibody, ES/130 antibody, ES130 antibody, FLJ36146 antibody, MGC157720 antibody, MGC157721 antibody, hES antibody, RRBP1 antibody, 180 kDa ribosome receptor homolog antibody, ribosome-binding protein 1 antibody, ribosome receptor protein antibody, ES/130-related protein antibody, ribosome binding protein 1 homolog 180kDa (dog) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
152 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1183 and 1410 of Ribosome binding protein 1 (Uniprot ID# Q9P2E9)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1(107-10294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Ribosome binding protein 1(107-10294) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:500.
Ribosome binding protein 1 antibody validation by siRNA knock-down.
Upperpanel: Ribosome binding protein 1 antibody 107-10294
Lower panel: Vinculin antibody
A. 30 µg HepG2 whole cell lysate/extract
B. 30 µg whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells
C. 30 µg whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells
5 % SDS-PAGE
Ribosome binding protein 1 antibody (107-10294) dilution: 1:500
Vinculin antibody dilution: 1:1000
Upperpanel: Ribosome binding protein 1 antibody 107-10294
Lower panel: Vinculin antibody
A. 30 µg HepG2 whole cell lysate/extract
B. 30 µg whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells
C. 30 µg whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells
5 % SDS-PAGE
Ribosome binding protein 1 antibody (107-10294) dilution: 1:500
Vinculin antibody dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1(107-10294) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A:NIH-3T3
5% SDS PAGE
107-10294 diluted at 1:1000
A:NIH-3T3
5% SDS PAGE
107-10294 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.