Rabbit Anti-Human Transglutaminase (TGM2) (Center K444) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P21980 |
| Gene Id | 7052 |
| Gene Symbols | TGM2 |
| Protein Name / Synonyms | Transglutaminase (TGM2) (Center K444) |
| Clonality | Polyclonal |
| Preparation | This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It has been identified as the autoantigen implicated in celiac disease.
Specificity
Other Names
TGM2; Protein-glutamine gamma-glutamyltransferase 2; Tissue transglutaminase; Transglutaminase C; Transglutaminase H; Transglutaminase-2
NCBI Accession #
NP_004604.2;NP_945189.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
77329
Recommended Dilutions
WB: 1:1000; IF: 1:10-50; IHC: 1:10-50
 | TGM2 Antibody (Center K444) (Cat# 102-17645) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow). |
 | Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444)(Cat# 102-17645).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.