Transmembrane protein 147 antibody [N1C1], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 10430 |
| Protein Name / Synonyms | Transmembrane protein 147 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MGC1936 antibody, NIFIE14 antibody, TMEM147 antibody, protein NIFIE 14 antibody, seven transmembrane domain protein antibody, transmembrane protein 147 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
25 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 37 and 131 of Transmembrane protein 147 (Uniprot ID# Q9BVK8)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Transmembrane protein 147(107-10728) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Transmembrane protein 147(107-10728) antibody at 1:500 dilution.
Sample(30 µg of whole cell lysate)
A:Raji
12% SDS PAGE
107-10728 diluted at 1:1500
A:Raji
12% SDS PAGE
107-10728 diluted at 1:1500
Sample (30 ug of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
Transmembrane protein 147 antibody
107-10728 diluted at 1:1000
A: NIH-3T3
12% SDS PAGE
Transmembrane protein 147 antibody
107-10728 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.