Rabbit Anti-Human TREF1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q96PN7 |
| Gene Id | 55809 |
| Gene Symbols | TRERF1 |
| Protein Name / Synonyms | TREF1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This TREF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1136-1164 amino acids from the C-terminal region of human TREF1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | FC, IF, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
TREF1 encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1.
Specificity
Other Names
TRERF1; BCAR2; RAPA; TREP132; Transcriptional-regulating factor 1; Breast cancer anti-estrogen resistance 2; Transcriptional-regulating protein 132; Zinc finger protein rapa; Zinc finger transcription factor TReP-132
NCBI Accession #
NP_277037.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
132256
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; FC: 1:10-50
 | Fluorescent image of MCF-7 cell stained with TREF1 Antibody (C-term)(Cat# 102-18243).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TREF1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).TREF1 immunoreactivity is localized to Nucleus significantly. |
 | Western blot analysis of TREF1 Antibody (C-term) (Cat# 102-18243) in K562 cell line lysates (35ug/lane). TREF1 (arrow) was detected using the purified Pab. |
 | TREF1 Antibody (C-term) (Cat# 102-18243) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.