TRIM23 antibody [N1], N-Terminus, 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 373 |
| Protein Name / Synonyms | TRIM23, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ARD1 antibody, ARFD1 antibody, RNF46 antibody, TRIM23 antibody, "ADP-ribosylation factor domain protein 1, 64kDa antibody", E3 ubiquitin-protein ligase TRIM23 antibody, ARF domain protein 1 antibody, ADP-ribosylation factor domain-containing protein 1 antibody, GTP-binding protein ARD-1 antibody, tripartite motif-containing 23 antibody, tripartite motif-containing protein 23 antibody, RING finger protein 46 antibody, tripartite motif protein TRIM23 antibody, tripartite motif containing 23 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
64 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 44 of TRIM23 (Uniprot ID# P36406)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23(107-10177) antibody (Green) at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded gastric CA, using TRIM23(107-10177) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:HeLa S3
10% SDS PAGE
107-10177 diluted at 1:1000
A:HeLa S3
10% SDS PAGE
107-10177 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.