IKBKB Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O14920 |
| Gene Id | 3551 |
| Gene Symbols | IKBKB |
| Protein Name / Synonyms | IKBKB, IKK-beta, IKK2, IKKB, IMD15, NFKBIKB, inhibitor of nuclear factor kappa-B kinase subunit beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
29kDa/79kDa/85kDa/86kDa
Observed MW
80kDa
Immunogen
A synthetic peptide of human IKBKB
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using IKBKB antibody (144-02087) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lung cancer using IKBKB antibody (144-02087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using IKBKB antibody (144-02087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using IKBKB antibody (144-02087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IKBKB antibody (144-02087) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IKBKB antibody (144-02087) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using IKBKB antibody (144-02087). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.