Phospho-LMNA-S22 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P02545 |
| Gene Id | 4000 |
| Gene Symbols | LMNA |
| Protein Name / Synonyms | LMNA, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1, lamin |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
62-74kDa
Observed MW
69kDa, 78kDa
Immunogen
A synthetic phosphorylated peptide around S22 of human LMNA (NP_005563.1).
Modification
Phospho S22
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-LMNA-S22 antibody (144-61515) at 1:2000 dilution or LMNA antibody (144-00249). HeLa cells were treated by Hydroxyurea (4 mM) at 37? for 20 hours or treated by Paclitaxel (100 nM/ml) at 37? for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37? for 20 hours or treated by Paclitaxel (100 nM) at 37? for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-LMNA-S22 pAb (144-61515). Western blot was performed from the immunoprecipitate using Phospho-LMNA-S22 pAb (144-61515) at a dilition of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37? for 20 hours.
Expiration:
12 months from the date of shipment when stored properly.