Phospho-NDRG1-S330 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q92597 |
| Gene Id | 10397 |
| Gene Symbols | NDRG1 |
| Protein Name / Synonyms | NDRG1, CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, protein NDRG1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
33kDa/35kDa/42kDa
Observed MW
50kDa
Immunogen
A synthetic phosphorylated peptide around S330 of human NDRG1 (NP_001128714.1).
Modification
Phospho S330
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat and C2C12 cells, using Phospho-NDRG1-S330 antibody (144-61531) at 1:2000 dilution. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.C2C12 cells were treated by Insulin (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.