Phospho-NEDD4L-S448 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96PU5 |
| Gene Id | 23327 |
| Gene Symbols | NEDD4L |
| Protein Name / Synonyms | NEDD4L, NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2, E3 ubiquitin-protein ligase NEDD4-like |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
96-111kDa
Observed MW
130kDa
Immunogen
A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1).
Modification
Phospho S448
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-NEDD4L-S448 antibody (144-61549) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.