Rabbit Anti-PRDX2 (C-term) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P32119 |
| Gene Id | 7001 |
| Gene Symbols | PRDX2 |
| Protein Name / Synonyms | PRDX2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human PRDX2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PRDX2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms.
Specificity
Predicted Reactivity
Rat, Bovine, Hamster, Monkey
Other Names
PRDX2; NKEFB; TDPX1; Peroxiredoxin-2; Natural killer cell-enhancing factor B; PRP; Thiol-specific antioxidant protein; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1
NCBI Accession #
NP_005800.3
Other Accession #
P35704; Q61171; Q2PFZ3; Q8K3U7; Q9BGI3
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
21892
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:50-100
 | Fluorescent confocal image of Hela cell stained with PRDX2 Antibody (C-term)(Cat# 102-15232).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PRDX2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).PRDX2 immunoreactivity is localized to Cytoplasm significantly. |
 | Western blot analysis of PRDX2 Antibody (C-term) (Cat# 102-15232) in MDA-MB231,293 cell line and mouse brain tissue lysates (35ug/lane).PRDX2 (arrow) was detected using the purified Pab.(2ug/ml) |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.