Phospho-RPS6KA5-S376 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75582 |
| Gene Id | 9252 |
| Gene Symbols | RPS6KA5 |
| Protein Name / Synonyms | RPS6KA5, MSK1, MSPK1, RLPK, ribosomal protein S6 kinase A5 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
61kDa/81kDa/89kDa
Observed MW
90kDa
Immunogen
A synthetic phosphorylated peptide around S376 of human RPS6KA5 (NP_004746.2).
Modification
Phospho S376
Storage
-20°C
Images
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RPS6KA5-S376 antibody (144-61526) at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin (25ug/ml) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.