RUVBL1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q9Y265 |
| Gene Id | 8607 |
| Gene Symbols | RUVBL1 |
| Protein Name / Synonyms | RUVBL1, ECP-54, ECP54, INO80H, NMP 238, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A, ruvB-like 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:20 - 1:50
Format
Calculated MW
42kDa/50kDa
Observed MW
55kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RUVBL1 (NP_003698.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RUVBL1 antibody (144-05723) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody (144-05723) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RUVBL1 Antibody (144-05723) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RUVBL1 Antibody (144-05723) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using RUVBL1 Antibody (144-05723) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using RUVBL1 antibody (144-05723). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.