Phospho-Stat3-S727 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P40763 |
| Gene Id | 6774 |
| Gene Symbols | STAT3 |
| Protein Name / Synonyms | STAT3, ADMIO, ADMIO1, APRF, HIES, signal transducer and activator of transcription 3 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.01% thimerosal, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
83kDa/87kDa/88kDa
Observed MW
86kDa
Immunogen
A synthetic phosphorylated peptide around S727 of human Stat3 (NP_644805.1).
Modification
Phospho S727
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-Stat3-S727 antibody (144-61497) at 1:1000 dilution or STAT3 antibody (144-01192). NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37? for 30 minutes or treated by UV at room temperature for 15-30 minutes. C6 cells were treated by Anisomycin (25 ug/ml) at 37? for 20 minutes or treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using Phospho-Stat3-S727 antibody (144-61497) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using Phospho-Stat3-S727 antibody (144-61497) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-Stat3-S727 antibody (144-61497) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-Stat3-S727 antibody (144-61497) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Phospho-Stat3-S727 antibody (144-61497) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Stat3-S727 pAb (144-61497). Western blot was performed from the immunoprecipitate using Phospho-Stat3-S727 pAb (144-61497) at a dilition of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37? for 15 minutes after serum-starvation overnight.
Expiration:
12 months from the date of shipment when stored properly.