TWIST1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15672 |
| Gene Id | 7291 |
| Gene Symbols | TWIST1 |
| Protein Name / Synonyms | TWIST1, ACS3, BPES2, BPES3, CRS, CRS1, CSO, SCS, TWIST, bHLHa38, twist-related protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
20kDa
Observed MW
21kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TWIST1 (NP_000465.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of U-87MG cells, using TWIST1 antibody (144-61361) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using TWIST1 antibody (144-61361) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TWIST1 antibody (144-61361) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.