Rabbit Anti-UBE2G1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P62253 |
| Gene Id | 7326 |
| Gene Symbols | UBE2G1 |
| Protein Name / Synonyms | UBE2G1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This UBE2G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2G1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2G1 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 98-100% sequence identity with the zebrafish, frog, rat and mouse counterparts, which indicates that this enzyme is highly conserved in eukaryotes.
Specificity
Predicted Reactivity
Rat, Monkey
Other Names
UBE2G1; UBE2G; Ubiquitin-conjugating enzyme E2 G1; E217K; UBC7; Ubiquitin carrier protein G1; Ubiquitin-protein ligase G1
NCBI Accession #
NP_003333.1
Other Accession #
P62255; P62254; Q4R5Y8; NP_003333
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
19509
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of anti-UBE2G1 Antibody (N-term) (Cat# 102-14977) in mouse heart tissue lysates (35ug/lane). UBE2G1(arrow) was detected using the purified Pab. |
 | Western blot analysis of UBE2G1 (arrow) using rabbit polyclonal UBE2G1 Antibody (R11) (Cat# 102-14977). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2G1 gene. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.