Rabbit IL-1 beta ELISA

RayBio® Rabbit IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$361.80
You save: $40.20 (10%)
ELL-IL1b-1
+

Antigen Information

Accession Number:
  • P14628
Gene ID:
  • 100008990
Gene Symbols:
  • IL1B
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta) (Lymphocyte proliferation-potentiating factor)
Target Species:
  • Rabbit

Assay Format

Detection Range:
45 pg/ml - 12,000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
45 pg/ml

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Specificity:
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rabbit IL-1alpha, IL-8, IL-17A, IL-21, Leptin, MIP-1 beta, MMP-9, NCAM-1, TNF alpha
Number of Targets Detected:
1
Species Detected:
  • Rabbit
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
The standard was easy to make and the protocol was easy to follow. Because rabbit ELISAs are hard to find it's good to know that there is a kit that works for us. We used this kit for plasma and other fluids.

University of Texas Health Science Center at Tyler
Reliable, useful and reproducible data. For our samples, we didn't find good kits in recent times and this kit really helped and saved lot of time. Simple protocol with a lot less instructions to follow. 2.5 hours of sample and standard incubation time could be less. Overall, happy with this kit and in fact, suggested this kit to my other fellow researchers!!!

University of Texas Health Science Center atTyler
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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