Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
11b-HSD1, 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ACE-2, ACTC1, Activin R1B (ALK-4), Activin R2A, ADAMTS-1 (METH1), ADAMTS15, ADAMTS-4, ADAMTS5, Adiponectin (ACRP30), Aggrecan, AHCY, AK1, ALDH1A1, ALK-1, Alpha 1 AG, Alpha-synuclein, AMPKa1, AMSH (STAMBP), Angiopoietin-1, Angiopoietin-2, Angiogenin, ANGPTL2, ANGPTL3, Annexin A5, Annexin A7, ApoA, ApoA2, ApoB, Artemin, B3GNT1, bax, beta-I Tubulin, BLAME (SLAMF8), BLC (CXCL13), BLMH, BMP-1 (PCP), BMP-2, BMP-3, BMP-4, BMP-6, BMP-9, ALK-6 (BMPR-IB), BMPR-II, BRCA2, C1QA, C1QC, C3a, C5a, CA1, CA3, CA9, Cadherin-6 (KCAD), CALD1, CapG, Caspase-14, Catalase, Cathepsin A, Cathepsin B (Pro-Cathepsin B), Cathepsin G, CCL3, CCR1, CCR6, CCR7, CCR8, CCR9, CD109, CD133, ADP-Ribosyl Cyclase 2 (BST-1/CD157), CD23, CD30 (TNFRSF8), CD36 (SR-B3), CD38, CD90 (Thy1), CD97, CD98, CDSN, Cerberus 1, Complement Factor H-related 5 (CFHR5), Chemerin, Chitotriosidase, Chymase, CKB, CK-MB, Claudin-4, CLC, Complement MASP3, COL19A1, COL1A1, Contactin-1, Contactin-4 (BIG-2), Cortactin, Carboxypeptidase A1 (CPA1), CRHBP, CRTAC1, CRTAM, Cardiotrophin-1 (CT-1), CTLA-4 (CD152), CXCL3, MIG (CXCL9), CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, Cyclin D1, Cyclophilin B, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, DAN, DDAH1, D-Dimer, Decorin, Desmocollin-2, Desmocollin-3, Desmoglein-2, DISC 1, DKK-1, Dystroglycan, E-Cadherin, EDA-A2, EDG-1, EG-VEGF (PK1), Endocan, Endoglin (CD105), Endorepellin (Perlecan), EN-RAGE (S100 A12), Eotaxin-1 (CCL11), TROP1 (EpCAM), EPCR, EphA1, EphA2, EphB6, Ephrin-A1, Epigen, Epiregulin, Erythropoietin R, ErbB2, ErbB4 (HER4), EVC2, EXTL2, FABP4 (A-FABP), Factor IX, Factor VII, Factor XI, Factor XII, FAM3C, Fas Ligand (TNFSF6), FASN, FGF Basic, FGF R4, FGF-10 (KGF-2), FGF-11, FGF-16, FGF-19, FGF-21, FGF-23, FGF-9, FGFR1 alpha, FGFR2, FGL2, Fibronectin, Fibulin 3, FKBP12, FKBP25, FKBP51, FLRG, Follistatin, Frizzled-4, Frizzled-6, FSH, Follistatin-like 1, FUCA1, G6PD, Galectin-3, GALNT2, GASP-1 (WFIKKNRP), Gastrokine 1, GCSF, GCSF R (CD114), GDF5, Gephyrin, GFR alpha-1 (GDNF R), GFR alpha-2, GFR alpha-3, Growth Hormone R, GLI-2, GLIPR2, GLO-1, Glut2, Glut3, Glut5, GPV (CD42d), Glypican 3, GM-CSF, GMF beta, gp340, GPD1, GPLD1, GPR-39, Grb2, GRP75, GRP78, H6PD, HABP2, HAI-1, Haptoglobin, HBB, HB-EGF, CGA, HEXB, Histone H1.3, Histone H3.3, HMGB1, hnRNPL, hnRNPU, HOXB3, HOXD11, HPRT, HRG, HRG-beta 1, HSP10, HSP90, HTRA1, HTRA2, ICAM-1 (CD54), ICAM-2 (CD102), ICAM-5, IDH1, IFNAR1, IFN-gamma, IFNGR1, IGF-2, IGFBP-1, IGFBP-6, IL-12RB2, IL-13, IL-16, IL-17A, IL-17F, IL-17RA, IL-17RB, IL-17RD, IL-19, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-36 alpha (IL-1 F6), IL-1 R1, IL-1 R2, IL-1 R3 (IL-1 R Acp), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-2, IL-20, IL-20RB, IL-21, IL-22, IL-22BP, IL-23, IL-23R, IL-26 (AK155), IL-27RA, IL-4, IL-6, IL-7, IL-7RA, IL-8 (CXCL8), IL-9 R (CD129), ILK, INSRR, Insulysin, Integrin beta 6, IP-10 (CXCL10), I-TAC (CXCL11), Kallikrein 5, Kallikrein 7, Kallikrein 8, KLKB1, KPNB1, LAMB2, Layilin, LBP, LECT2, LEDGF, Leptin, Leptin R, LIF, LIF R alpha, Lipocalin-2 (NGAL), LIX, LRP-6, LSAMP, L-Selectin (CD62L), LTB, Lymphotoxin beta R (TNF RIII), LYVE-1, Matrilin-3, Mcl-1, M-CSF, MDC (CCL22), Mer, Metavinculin, MFRP, MIP-1 beta (CCL4), MIP-3 beta (CCL19), MMP-10, MMP-13, MMP-16, MMP-2, MMP-7, MMP-8, MMP-9, MP1, Myeloperoxidase, MRP 1, MSH6, MyBPC3, Myoglobin, NCAM-1 (CD56), Nectin-3, NET1, Netrin-4, Neurokinin A, Neuropilin-1, Neuropilin-2, Neurotrimin, Nidogen-2, Notch-1, Notch-3, NPTXR, NR3C3, NrCAM, OBCAM (OPCML), Olfactomedin-1, OSCAR, Oncostatin M R beta (IL-31 R beta), Osteoactivin (GPNMB), Osteoadherin (Osteomodulin), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), P20Sb3, p27/Kip1 (CDKN1B), P4HB, p53, PAK7, PCNA, PCPE-1, Proprotein Convertase 9 (PCSK9), PDAP1, PDGF R beta, PDGF-BB, PDGF-C, PD-L1, PDX-1 (IPF1), PEBP1, Pentraxin-3 (TSG-14), Periostin, Platelet Factor 4 (CXCL4), PFAS, PGM1, PGRPs, PHGDH, PLA2G1B, Plasminogen, PLOD2, PNP, Podocalyxin, POR, PP, PRCP, Pref-1 (DLK-1/FA1), PREP, Presenilin 1, Procalcitonin, Prolactin, Prolargin, Prostasin, Coagulation Factor XIV (Protein C), PSMA1, PTEN, PTH, PTP gamma, PTP mu, PTPRZ, RANTES (CCL5), Ras, RBP4, Reg1A, RELT (TNFRSF19L), Resistin, ROBO4, ROCK2, RREB1, S100 A1, S100A10, S100A11, S100A9, SCF, SCF R (CD117/c-kit), SCGF (CLEC11a), Serpin A12, Serpin A3, Serpin B4, Serpin B5, Serpin B8, Serpin C1, Serpin D1, SERPINF1 (PEDF), SERPINF2 (Alpha-2-Antiplasmin), SerRS, sFRP-4, SHP-1, Siglec-1 (CD169), SLITRK1, Smad 1, Smad 4, Smad 5, Smad 7, SMPD4, SOD2, SOD3, Somatostatin, SorLA, SOST, SOX2, SPARC (Osteonectin), SP-D, Src, SREC-II (SCARF2), SSEA-1, SSTR2, SVEP1, TCP1 eta, TFF1, TGF beta 3, TGF beta R2, TGF beta R3, TGF alpha, TGF beta 2, TGF-beta R1, Thioredoxin-1, Thrombopoietin (TPO), TIM-4, TIMP-1, TIMP-2, TL1A, TLR1, TLR2, TLR3, TLR4, TMEFF2, TNF alpha, TOP2B, Tripeptidyl-peptidase I (TPP1), TRADD, TRANCE (TNFSF11), TRAP220, TROY (TNFRSF19), TSG-6, TSH, Thrombospondin 1, Thrombospondin-2, TSP-4, TWEAK (TNFSF12), TWEAK R (TNFRSF12), TXNDC15, TXNDC5, Ubiquitin+1, UCH-L1, UNC5H4, uPA, uPAR, UROC1, UROD, USP14, USP2, Uteroglobin (SCGB1A1), VAP-1 (AOC3), VARS, VE-Cadherin (CDH5), VEGF-A, VEGF-C, VEGFR1, VEGFR2, VEGFR3, Versican, VILIP3, Vimentin B, Vitronectin, VSIG4, WISP-1 (CCN4), XIAP, ZC3H4
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Membranes
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Detection Buffer C
- Detection Buffer D
- Wash Buffer 1
- Wash Buffer 2
- HRP-Conjugated Streptavidin Concentrate
- Plastic Sheets
- Spin Columns
- Labeling Buffer
- Lysis Buffer
- Plastic Incubation Tray
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- 2?5 ml tube
- 15 ml conical collection tube
- Tissue Paper, blotting paper or chromatography paper
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Block array membranes
- Incubate with Samples
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.