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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Rat |
| Sensitivity | 17 pg/ml |
| Detection Range | 17 pg/ml - 4000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat beta-NGF, CD86 (B7-2), CINC-1 (CXCL1), CINC-3, CNTF, Fractalkine (CX3CL1), GM-CSF, ICAM-1 (CD54), IFNgamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-10, IL-13, IL-2, IL-4, IL-6, LIX, L-Selectin (CD62L), MCP-1 (CCL2), PDGF-AA, Prolactin R, RAGE, TCK-1 (CXCL7), TIMP-1, TNF alpha, VEGF-A. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | Q10746 |
| Gene Id | 171551 |
| Gene Symbols | CXCL3, CINC2 |
| Protein Name / Synonyms | C-X-C motif chemokine 3 (Cytokine-induced neutrophil chemoattractant 2) (CINC-2) (Macrophage inflammatory protein 2-alpha/beta) (MIP2-alpha/beta) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
