RayBio® Rat IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • P20607
Gene ID:
  • 24498
Gene Symbols:
  • IL-6
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6)
Target Species:
  • Rat

Assay Format

Detection Range:
30 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
30 pg/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Dallak, Mohammad. "A synergistic protective effect of selenium and taurine against experimentally induced myocardial infarction in rats."?Archives of physiology and biochemistry?123.5 (2017): 344-355.
    Species: Rat
    Sample type: Tissue Lysate
  2. Dokubo, A., A. A. Uwakwe, and B. A. Amadi. "A study on the antioxidant and anti-inflammatory effects of aframomum sceptrum and parinari congensis seed extracts in alloxan-induced diabetic wistar rats."?EPRA International Journal of Research & Development?2.7 (2017): 1-9.
    Species: Rat
    Sample type: Tissue
  3. Dora, Chander Parkash, et al. "Improved metabolic stability and therapeutic efficacy of a novel molecular gemcitabine phospholipid complex."?International journal of pharmaceutics?530.1-2 (2017): 113-127.
    Species: Rat
    Sample type: Serum
  4. Gu Y., He M., Zhou X., et al. Endogenous IL-6 of mesenchymal stem cell improves behavioral outcome of hypoxic-ischemic brain damage neonatal rats by supressing apoptosis in astrocyte. Sci Rep. 2016 Jan 14;6:18587. doi: 10.1038/srep18587.
    Species: Rat
    Sample type: Tissue Lysate
  5. Xu B., Yang H., Sun M., et al. 2,3',4,4',5-Pentachlorobiphenyl Induces Inflammatory Responses in the Thyroid Through JNK and Aryl Hydrocarbon Receptor-Mediated Pathway. Toxicol Sci. 2016 Feb;149(2):300-11. doi: 10.1093/toxsci/kfv235.
    Species: Rat
    Sample type: Conditioned Media
  6. Li P., Liu H., Sun P., et al. Chronic vagus nerve stimulation attenuates vascular endothelial impairments and reduces the inflammatory profile via inhibition of the NF-?B signaling pathway in ovariectomized rats. Exp Gerontol. 2016 Feb;74:43-55. doi: 10.1016/j.exger.2015.12.005.
    Species: Rat
    Sample type: Serum
  7. Suchal, Kapil, et al. "Mangiferin protect myocardial insults through modulation of MAPK/TGF-? pathways."?European journal of pharmacology?776 (2016): 34-43.
    Species: Rat
    Sample type: Serum
  8. Abdel kawy H. Low-dose carvedilol protects against acute septic renal injury in rats during the early and late phases. Can J Physiol Pharmacol. 2015 Jun;93(6):443-50. doi: 10.1139/cjpp-2014-0516
    Species: Rat
    Sample type: Serum
  9. Aydin A., Halici Z., Albayrak A., et al. Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions. Basic Clin Pharmacol Toxicol. 2015 Jan 27. doi: 10.1111/bcpt.12384
    Species: Rat
    Sample type: Serum
  10. Jain V., Pareek A., Ratan Y., Singh N. Standardized fruit extract of Momordica charantia L protect against vincristine induced neuropathic pain in rats by modulating GABAergic action, antimitotoxic, NOS inhibition, anti-inflammatory and antioxidative activity. South African Journal of Botany. Volume 97, March 2015, Pages 123-132
    Species: Rat
    Sample type: Tissue Lysate
The kit is wonderful, I used Kidney homogenates.

University of Houston
Excellent results; easy to use.

SUNY Downstate Medical Center
Work really well on rat adipose tissue

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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