Rat IL-6 ELISA

Old price: $402.00
$361.80
You save: $40.20 (10%)

RayBio® Rat IL-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELR-IL6-1
+

Antigen Information

Accession Number:
  • P20607
Gene ID:
  • 24498
Gene Symbol:
  • IL-6
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6)
Target Species:
  • Rat

Assay Format

Detection Range:
30 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
30 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
1
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Gu Y., He M., Zhou X., et al. Endogenous IL-6 of mesenchymal stem cell improves behavioral outcome of hypoxic-ischemic brain damage neonatal rats by supressing apoptosis in astrocyte. Sci Rep. 2016 Jan 14;6:18587. doi: 10.1038/srep18587.
    Species: Rat
    Sample type: Tissue Lysate
  2. Xu B., Yang H., Sun M., et al. 2,3',4,4',5-Pentachlorobiphenyl Induces Inflammatory Responses in the Thyroid Through JNK and Aryl Hydrocarbon Receptor-Mediated Pathway. Toxicol Sci. 2016 Feb;149(2):300-11. doi: 10.1093/toxsci/kfv235.
    Species: Rat
    Sample type: Conditioned Media
  3. Li P., Liu H., Sun P., et al. Chronic vagus nerve stimulation attenuates vascular endothelial impairments and reduces the inflammatory profile via inhibition of the NF-?B signaling pathway in ovariectomized rats. Exp Gerontol. 2016 Feb;74:43-55. doi: 10.1016/j.exger.2015.12.005.
    Species: Rat
    Sample type: Serum
  4. Suchal, Kapil, et al. "Mangiferin protect myocardial insults through modulation of MAPK/TGF-? pathways." European journal of pharmacology 776 (2016): 34-43.
    Species: Rat
    Sample type: Serum
  5. Abdel kawy H. Low-dose carvedilol protects against acute septic renal injury in rats during the early and late phases. Can J Physiol Pharmacol. 2015 Jun;93(6):443-50. doi: 10.1139/cjpp-2014-0516
    Species: Rat
    Sample type: Serum
  6. Aydin A., Halici Z., Albayrak A., et al. Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions. Basic Clin Pharmacol Toxicol. 2015 Jan 27. doi: 10.1111/bcpt.12384
    Species: Rat
    Sample type: Serum
  7. Jain V., Pareek A., Ratan Y., Singh N. Standardized fruit extract of Momordica charantia L protect against vincristine induced neuropathic pain in rats by modulating GABAergic action, antimitotoxic, NOS inhibition, anti-inflammatory and antioxidative activity. South African Journal of Botany. Volume 97, March 2015, Pages 123-132
    Species: Rat
    Sample type: Tissue Lysate
  8. Shah A., Hagawane T., Kshirsagar N., Banerjee R. Surface-active drug loaded lipopolymeric nanohybrid aerosol therapy: potential non-invasive way to mitigate lipopolysaccharide mediated inflammation in murine lungs . RSC Advances 2015, 5, 9683 DOI: 10.1039/c4ra13558j
    Species: Rat
    Sample type: BAL Fluid
  9. Vashistha B., Sharma A., Jain V. Ameliorative potential of ferulic acid in vincristine-induced painful neuropathy in rats: An evidence of behavioral and biochemical examination. Nutr Neurosci. 2014 Dec 15. [Epub ahead of print] http://dx.doi.org/10.1179/1476830514Y.0000000165
    Species: Rat
    Sample type: Tissue Lysate
  10. Yaidikur L., Thakur S. Punicalagin attenuated cerebral ischemia-reperfusion insult via inhibition of proinflammatory cytokines, up-regulation of Bcl-2, down-regulation of Bax, and caspase-3. Mol Cell Biochem. 2015 Apr;402(1-2):141-8. doi: 10.1007/s11010-014-2321-y.
    Species: Rat
    Sample type: Tissue Lysate
Work really well on rat adipose tissue
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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