Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
11b-HSD1, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 sigma, 14-3-3 theta, 4-1BB (TNFRSF9/CD137), A2B5, ACACA, ACTC1, Actin, Activin R2A, ADAM10, ADAMTS-1 (METH1), Adiponectin (ACRP30), aFGF (FGF-1), Agrin, AIF, AK1, ALCAM (CD166), ALK-7 (ACVR1C/Activin A Receptor), Alpha-Actinin 1, Alpha-Galactosidase A (GLA), Alpha-Synuclein, Ameloblastin, AMPK alpha 2, Androgen R, ANGPT1, Annexin A1, Annexin A4, Annexin A7, Annexin V, APE1 (APEX1), APRIL, Arginase 1, ART4, ASAH2, B3GNT2, BAFF, BAK (Bcl2-L-7), BCAM, BCL-10, Bcl-2, Bcl-w, Bcl-xL, beta 2-Microglobulin, beta IG-H3, bFGF, BID, BIK, BLVRB, BMP-2, BMP-7, B-raf, BST1, BTLA (CD272), C4.4A (LYPD3), Cadherin-4, Calcineurin A, Calcineurin B, Carboxylesterase 1 (CES1), Carboxypeptidase A1 (CPA1), Carboxypeptidase B1 (CPB1), Caspr 2, Catalase, Cathepsin B (Pro-Cathepsin B), Cathepsin C, Cathepsin E, Cathepsin L, Cathepsin X, Caveolin-2, CCK-A R, CCL26-Like, CD13, CD14, CD155 (PVR), CD1d1, CD200, CD22, CD226, CD27 (TNFRSF7), CD276, CD300f (CLM-1/IREM-1), CD300LG, CD31, CD34, CD38, CD39L1, CD4, CD47, CD48 (SLAMF2), CD5L, CD6, CD63, CD68, CD79B, CD8 alpha, CD83, CD86 (B7-2), CD93, CDC25B, CDC37, CDH2, CDNF, CHMP2B, Chordin, CIB1 (CIBP), CLEC4A2, CLEC4B2, Clusterin, CNTF, CO5, Coagulation Factor XIV (Protein C), COLEC12, Complexin-2, Contactin-1, Contactin-2 (TAG1), Contactin-4 (BIG-2), Cortactin, CPA2, CRELD1, CRELD2, CrkL, CRP (C-Reactive Protein), CRYAB, CSF1R, CTACK (CCL27), CTGF, CTHRC1, CTLA-4 (CD152), Cubilin, CXCL10, CXCL16, Cyclophilin A, Cyclophilin B, Cystatin C, Cytochrome C, Decorin, DEP-1, DGK-epsilon, DHFR, DKK-3, DLL1, DOK7, DYRK2, DYRK3, E-Cadherin, ECM1, EEA1, EFNA1, EFNB1, EGF, EGFR, eIF5A, EMP, ENO1, Eotaxin-1 (CCL11), EphA5, EphB1, EphB6, Ephrin-A2, Ephrin-B2, ER alpha (NR3A1), ErbB2, ErbB3, Erythropoietin, Ets-1, Ezrin, F2, F3, FABP1, FABP2, FABP3, FABP4 (A-FABP), FABP5 (E-FABP), FAK, FCAR (CD89), FCGR1, FETUB, FGF-12, FGF-21, FGFR4, Fgr, Fibromodulin, FKBP12, FKBP12.6, FKBP13, FKBP25, FKBP51, FKBP52, FLIP, FLT1, Flt-3 Ligand, Follistatin, FOLR1, FRK, FRS2, GABRA4, GAD1, Galectin-1, Galectin-3, Galectin-4, GAPDH, Gas 1, GDF-3, GDF-8 (Myostatin), GDNF, GFAP, GFRA1, GFRA2, GFRA3, GGT1, GIT1, GITR (TNFRSF18), GLG1, GLRX3, Glyoxalase 1, Glyoxalase 2, Glypican 3, gp130 (sgp130), GPT, Gpt2, GPX1, GPX3, Granzyme B, GRB2, GRIN2A, GRK1, GRK2, GRK5, GRO alpha (CXCL1), Growth Hormone, GRP75, HAAO, HABP1, HGF, HIF-1 alpha, HO-2, HPRG, HPX, HSP20, HSP27, HSP40, HSP60, HSP70, HSP90, HSPA8, HSPH1, HTRA2, Human Amyloid-like protein 1 (APLP-1), Iduronate 2-Sulfatase (IDS), IFN alpha, IFN alpha 5, IFN-gamma, IFN-gamma R2, IGF-1, IGFBP-5, IGSF4B (SynCAM3/CADM3), IGSF8 (CD316), IkB-beta, IKK, IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 Ra (IL-1 F3), IL-10, IL-11 R alpha, IL-12 p70, IL-13 R alpha 2, IL-15 R alpha, IL-17 RC, IL-18, IL-18 BPc, IL-2, IL-2 R alpha, IL-2 R gamma (Common gamma Chain), IL-21, IL-22, IL-23 p19, IL-31, IL-4, IL-4 R, IL-6, IL-7, IL-7 R alpha (CD127), IL-9, IL-9 R (CD129), ILK, ILKAP, IMPDH1, IPP2, Islet-1, Jagged 1, JAM-A (CD321/F11R), JAM-C (CD323), JNK1, JNK2 (MAPK9 isoform alpha-1), KDR, Keap1, Kininogen (Kininostatin), KIRREL3 (NEPH2), KLKB1, Lactoferrin, LAIR1, LAR, LAYN, LDHA, Legumain, Leptin, Leptin Receptor, LIF, LIFR, LILRA5 (ILT-11/CD85f), LILRC2, Lipocalin-2 (NGAL), LMW-PTP (ACP-1), LPHN3, LRP-4, Lymphotoxin beta R (TNF RIII), Lyn, MAG, Matrilin-3, MBL-2, MCAM (CD146), MCP-3 (MARC/CCL7), MEK2, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-3 beta (CCL19), MKK6 (MEK6), MMP-2, MMP-8, MMP-9, MOG, MP1, MST1, Myeloperoxidase, NCAM-1 (CD56), NCR3, NEDD4, NEDD8, Nephrin, Nestin, Netrin-1, Neurexophilin-1, Neuritin, Neurocan, Neurofascin, Neurogranin, Neuroligin-1, Neuroligin-2, Neuropilin-1, Neuroplastin 65, NFATC3, NF-L, NM23-H1/H2, nNOS, NNUP85, Noggin, Nogo-A, Nope, Notch-1, Notch-2, Notch-3, NPC2, NRAGE, Nrf2, NRXN1 beta, Olfactomedin-1, OLR1, Osteocalcin, OX40 (TNFRSF4), p27/Kip1 (CDKN1B), p38 gamma, p53, PAK1, PAK7, Pax7, P-Cadherin, PCDH-17, PCK1, PDGF R alpha, PDGF-BB, Pentraxin-2, Peroxiredoxin 6, PFKM, PGC, PI3-Kinase p55-gamma, pIgR, PIM2, PKA C a/b, PKC, PKC a, PKC i/l/z, PKM2, PLAUR, Plexin A4, PON3, POR, PP2A CS, PP2C alpha, PPA1, PPP2R4, PRDX 2, PRDX1, PRDX4, Pref-1 (DLK-1/FA1), PRL-3, PRL8A4, PROCR, Prolactin, Properdin, PSAP, PSMA1, PSMA2, PTK7, PTP1B, PVRL2, RalA, RALT, RANTES (CCL5), RBBP4, RBP4, Reg III, Reg3B, Renin 1, RHD, ROBO1, ROCK2, Secretagogin, Semaphorin 4C, Semaphorin 4D, Semaphorin 5A, Semaphorin 7A, Serpin A1, Serpin E1, Serpin F1, Serpin F2 (Alpha-2-Antiplasmin), SH2B1, SHIP2, SHP-1, SIGNR1 (CD209b), SIRP alpha, SLAMF1, SLC4A1, Slit3, Smad 3, Smad 7, SMC1, Sortilin, SOST, SOX1, SOX10, SOX2, SP-D, Src, STAT3, Syndecan-1, Syndecan-2, Syntaxin 1A, TAFA5, Talin1, TCK-1 (CXCL7), TC-PTP, TDP-43, TGF beta R3, THBD, Thioredoxin-2, Tie-2, TIM-1 (KIM-1), TNF alpha, TNF-R1, TNFRSF11A, TNFSF9, Tollip, TPT1, TRAF-2, TRAF-3 Isoform 2, Transferrin, Transgelin, Transglutaminase 2 (TGM2), TREM-1, TRHDE, TrkA, TRKB, TRKC, TWEAK R (TNFRSF12), UCH-L1, UCH-L3, Unc5h1, Unc5h2, VAMP-2, VHR, Vinculin, VSIG1, WFDC2, Wnt5a, XIAP, XPNPEP2, Zyxin
Target Species
Rat
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Membranes
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Detection Buffer C
- Detection Buffer D
- Wash Buffer 1
- Wash Buffer 2
- HRP-Conjugated Streptavidin Concentrate
- Plastic Sheets
- Spin Columns
- Labeling Buffer
- Lysis Buffer
- Plastic Incubation Tray
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- 2?5 ml tube
- 15 ml conical collection tube
- Tissue Paper, blotting paper or chromatography paper
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Block array membranes
- Incubate with Samples
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.