Rat MCP-1 ELISA

RayBio® Rat MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELR-MCP1-1
+

Antigen Information

Accession Number:
  • P14844
Gene ID:
  • 24770
Gene Symbols:
  • CCL2
  • JE
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (Immediate-early serum-responsive protein JE) (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2)
Target Species:
  • Rat

Assay Format

Detection Range:
15 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
500 - 10,000 fold
Sensitivity:
15 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Li P., Liu H., Sun P., et al. Chronic vagus nerve stimulation attenuates vascular endothelial impairments and reduces the inflammatory profile via inhibition of the NF-?B signaling pathway in ovariectomized rats. Exp Gerontol. 2016 Feb;74:43-55. doi: 10.1016/j.exger.2015.12.005.
    Species: Rat
    Sample type: Serum
  2. Luo Q., Wang XL., Di L., et al. Isolation and identification of renoprotective substances from the mushroom Ganoderma lucidum. Tetrahedron 71 (2015) 840 - 845 doi:10.1016/j.tet.2014.12.052
    Species: Rat
    Sample type: Conditioned Media
  3. Zhang Y., Zhang S., Tsui N. Mesenteric Lymph Duct Drainage Attenuates Acute Lung Injury in Rats with Severe Intraperitoneal Infection. Inflammation, Vol. 38, No. 3, June 2015, p1239 - 1249. DOI: 10.1007/s10753-014-0091-z
    Species: Rat
    Sample type: Tissue Lysate
  4. Yan YM., Fang P., Yang MT., et al. Anti-diabetic nephropathycompoundsfrom Cinnamomum cassia. J Ethnopharmacol. 2015 May 13;165:141-7. doi: 10.1016/j.jep.2015.01.049.
    Species: Rat
    Sample type: Conditioned Media
  5. Kaur H., Patro I., Tikoo K., Sandhir R. Curcumin attenuates inflammatory response and cognitive deficits in experimental model of chronic epilepsy. Neurochem Int. 2015 Oct;89:40-50. doi: 10.1016/j.neuint.2015.07.009
    Species: Human
    Sample type: Tissue Lysate
  6. Ikutomi M., Sahara M., Nakajima T., et al. Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation. J Mol Cell Cardiol. 2015 Sep;86:121-35. doi: 10.1016/j.yjmcc.2015.07.019.
    Species: Rat
    Sample type: Conditioned Media
  7. Wu C., Lv C., Chen F., et al. The function of miR-199a-5p/Klotho regulating TLR4/NF-?B p65/NGAL pathways in rat mesangial cells cultured with high glucose and the mechanism. Mol Cell Endocrinol. 2015 Dec 5;417:84-93. doi: 10.1016/j.mce.2015.09.024.
    Species: Rat
    Sample type: Conditioned Media
  8. Kovalska J., Mishra R., Jebavy L., et al. Tumour Progression and Spontaneous Regression in the Lewis Rat Sarcoma Model. Anticancer Res. 2015 Dec;35(12):6539-49.
    Species: Rat
    Sample type: Serum
  9. Alique M., Sanchez-Lopez E., Rayego-Mateos S., et al. Angiotensin II, via angiotensin receptor type 1/nuclear factor-?B activation, causes a synergistic effect on interleukin-1-?-induced inflammatory responses in cultured mesangial cells. J Renin Angiotensin Aldosterone Syst. 2014 Oct 29. pii: 1470320314551564
    Species: Rat
    Sample type: Cell Lysate
  10. Hsu YJ., Wang LC., Yang WS., et al. Effects of Fenofibrate on Adiponectin Expression in Retinas of Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research Volume 2014, Article ID 540326, 14 pages http://dx.doi.org/10.1155/2014/540326
    Species: Rat
    Sample type: Plasma
Excellent results with rat tissue homogenates and serum; easy to use; reproducible

SUNY Downstate Medical Center
Worked well for both plasma and tissue homogenate.

University of Houston
Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

Customers Also Bought Customers Also Bought