RayBio® Rat VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • P16612
Gene ID:
  • 83785
Gene Symbols:
  • VEGF
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Rat

Assay Format

Detection Range:
2 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
2 fold
2 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Patyar, Sazal, et al. "Chemopreventive effect of artesunate in 1, 2-dimethylhydrazine-induced rat colon carcinogenesis."?Journal of advanced pharmaceutical technology & research8.3 (2017): 102.
    Species: Rat
    Sample type: plasma
  2. El-Mahdy N., Salem M., El-Sayad M., et al. Bone marrow mononuclear cells enhance anti-inflammatory effects of pravastatin against isoproterenol-induced myocardial infarction in rats. J Immunotoxicol. 2016 May;13(3):393-402. doi: 10.3109/1547691X.2015.1111959.
    Species: Rat
    Sample type: Tissue lysate
  3. Kaya MK., Demir T., Bulut H., et al. Effects of lapatinib and trastuzumab on vascular endothelial growth factor in experimental corneal neovascularization. Clin Experiment Ophthalmol. 2015 Feb 2. doi: 10.1111/ceo.12500.
    Species: Rat
    Sample type: Tissue Lysate
  4. Lin F., Pan LH., Ruan L., et al. Differential expression of HIF-1?, AQP-1, and VEGF under acute hypoxic conditions in the non-ventilated lung of a one-lung ventilation rat model. Life Sci. 2015 Mar 1;124:50-5. doi: 10.1016/j.lfs.2014.12.020.
    Species: Rat
    Sample type: Tissue Lysate
  5. Mohammadpour A., Tavassoli A., Khakzad M., et al. Effect of gold nanoparticles on postoperative peritoneal adhesions in rats. Article 6, Volume 2, Issue 3, Summer 2015, Page 211-216. DOI: 10.7508/nmj.2015.03.006
    Species: Rat
    Sample type: Serum
  6. Ikutomi M., Sahara M., Nakajima T., et al. Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation. J Mol Cell Cardiol. 2015 Sep;86:121-35. doi: 10.1016/j.yjmcc.2015.07.019.
    Species: Rat
    Sample type: Conditioned Media
  7. Yu Y., Zou J., Han Y. Effects of intravitreal injection of netrin-1 in retinal neovascularization of streptozotocin-induced diabetic rats. Drug Des Devel Ther. 2015; 9: 6363-6377. doi: 10.2147/DDDT.S93166
    Species: Rat
    Sample type: Tissue Lysate
  8. Adamek B., et al. Vascular endothelial growth factor (VEGF) liver tissue concentration in delayed stages of rat liver regeneration upon interferon a2b influence. Post?py Nauk Medycznych. t. XXVII, nr 6, 2014
    Species: Rat
    Sample type: Tissue Lysate
  9. Hao C., Huang J., Shi Y., Cheng X., Li H., Zhou L., et al. Pulsed electromagnetic field improves cardiac function in response to myocardial infarction. Am J Transl Res 2014;6(3):281-290
    Species: Rat
    Sample type: Tissue Lysate
  10. Hao C., Huang Z., Song S., Shi Y., Cheng X., Murohara T., Lu W., Su D., Duan J. Arterial Baroreflex Dysfunction Impairs Ischemia?Induced Angiogenesis .J Am Heart Assoc.2014; 3: e000804.
    Species: Rat
    Sample type: Tissue Lysate

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