Publications by RayBiotech Scientists

RayBiotech commercialized the first protein array in 2001, and our scientists have published on antibody arrays, immunoassay development, and biomarker discovery ever since. Browse 109 peer-reviewed papers, reviews, and book chapters below, newest first. Names in bold are RayBiotech scientists, and each title links to the full paper.

Jump to: Research Articles (69) | Review Articles (24) | Methods and Book Chapters (16)

Research by year: 2026 · 2025 · 2024 · 2023 · 2022 · 2021 · 2020 · 2019 · 2018 · 2017 · 2016 · 2015 · 2014 · 2013 · 2012 · 2001-2011

2016

• Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). Zhou L, Lv Z, Shao J, Xu Y, Luo X, Zhang Y, ..., Hu Y, Zhang W, Luo S, Fang J, ..., Huang R. J Clin Lab Anal. 2016;30(5):581-9.
• Extracellular translationally controlled tumor protein promotes colorectal cancer invasion and metastasis through Cdc42/JNK/ MMP9 signaling. Xiao B, Chen D, Luo S, Hao W, Jing F, Liu T, ..., Ma Q. Oncotarget. 2016;7(31):50057-50073.
• Identification and characterization of monoclonal antibodies against GP73 for use as a potential biomarker in liver cancer screening and diagnosis. Zhang Y, Xi Y, Fang J, Luo S, Wilson JJ, Huang RP. J Immunoassay Immunochem. 2016;37(4):390-406.
• Network-driven plasma proteomics expose molecular changes in the Alzheimer's brain. Jaeger PA, Lucin KM, Britschgi M, Vardarajan B, Huang RP, Kirby ED, ..., Huang R, ..., Wyss-Coray T. Mol Neurodegener. 2016;11:31.
• Selection of a Novel Aptamer Against Vitronectin Using Capillary Electrophoresis and Next Generation Sequencing. Stuart CH, Riley KR, Boyacioglu O, Herpai DM, Debinski W, Qasem S, Marini FC, Colyer CL, Gmeiner WH. Mol Ther Nucleic Acids. 2016;5(11):e386.

2015

• Association between Prediagnostic Allergy-Related Serum Cytokines and Glioma. Schwartzbaum J, Seweryn M, Holloman C, Harris R, Handelman SK, Rempala GA, ..., Huang RP, Burkholder B, ..., Grimsrud TK. PLoS One. 2015;10(9):e0137503.
• Development of Monoclonal Antibodies and Characterization of an ELISA Platform Against Kallikrein-Related Peptidase 6 as a Tumor Biomarker. Xi Y, Zhang Y, Fang J, Wilson JJ, Luo S, Huang RP. Monoclon Antib Immunodiagn Immunother. 2015;34(5):346-53.

2014

• Protein expression profiling by antibody array analysis with use of dried blood spot samples on filter paper. Jiang W, Mao YQ, Huang R, Duan C, Xi Y, Yang K, Huang RP. J Immunol Methods. 2014;403(1-2):79-86.

2013

• Identification of five serum protein markers for detection of ovarian cancer by antibody arrays. Jiang W, Huang R, Duan C, Fu L, Xi Y, Yang Y, Yang WM, Yang D, Yang DH, Huang RP. PLoS One. 2013;8(10):e76795.

2012

• Development of IGF signaling antibody arrays for the identification of hepatocellular carcinoma biomarkers. Zhou Q, Mao YQ, Jiang WD, Chen YR, Huang RY, Zhou XB, Wang YF, Shi Z, Wang ZS, Huang RP. PLoS One. 2012;7(10):e46851.
• Enhanced chemosensitization in multidrug-resistant human breast cancer cells by inhibition of IL-6 and IL-8 production. Shi Z, Yang WM, Chen LP, Yang DH, Zhou Q, Zhu J, Chen JJ, Huang RC, Chen ZS, Huang RP. Breast Cancer Res Treat. 2012;135(3):737-47.
• Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis. Browne AS, Yu J, Huang RP, Francisco AM, Sidell N, Taylor RN. Fertil Steril. 2012;98(3):713-9.

2010

• A biotin label-based antibody array for high-content profiling of protein expression. Huang R, Jiang W, Yang J, Mao YQ, Zhang Y, Yang W, Yang D, Burkholder B, Huang RF, Huang RP. Cancer Genomics Proteomics. 2010;7(3):129-41.
• Multiple anticancer activities of EF24, a novel curcumin analog, on human ovarian carcinoma cells. Tan X, Sidell N, Mancini A, Huang RP, Shenming Wang, Horowitz IR, Liotta DC, Taylor RN, Wieser F. Reprod Sci. 2010;17(10):931-40.

2007

• Concurrent down-regulation of Egr-1 and gelsolin in the majority of human breast cancer cells. Liu J, Liu YG, Huang R, Yao C, Li S, Yang W, Yang D, Huang RP. Cancer Genomics Proteomics. 2007;4(6):377-85.
• Laminar shear inhibits tubule formation and migration of endothelial cells by an angiopoietin-2 dependent mechanism. Tressel SL, Huang RP, Tomsen N, Jo H. Arterioscler Thromb Vasc Biol. 2007;27(10):2150-6.

2006

• Early Growth Response-1 Suppresses Human Fibrosarcoma Cell Invasion and Angiogenesis. Huang R, Li S, Yang W, Chen L, Yao C, Huang RP. Cancer Genomics Proteomics. 2006;3(2):71-82.

2005

• Angiogenic effect of interleukin-8 in breast cancer and its association with estrogen receptor. Lin Y, Wang SM, Huang RP. Zhonghua Yi Xue Za Zhi. 2005;85(20):1419-23.
• Effect of interleukin-8 in cell invasion and proliferation of human breast cancer. Lin Y, Wang SM, Lü WM, Huang RP. Zhonghua Wai Ke Za Zhi. 2005;43(23):1541-4.

2004

• Enhanced protein profiling arrays with ELISA-based amplification for high-throughput molecular changes of tumor patients' plasma. Huang R, Lin Y, Shi Q, Flowers L, Ramachandran S, Horowitz IR, Parthasarathy S, Huang RP. Clin Cancer Res. 2004;10(2):598-609.
• Identification of interleukin-8 as estrogen receptor-regulated factor involved in breast cancer invasion and angiogenesis by protein arrays. Lin Y, Huang R, Chen L, Li S, Shi Q, Jordan C, Huang RP. Int J Cancer. 2004;109(4):507-15.
• Molecular Profiling of Circulating Cytokine Levels in Human Ovarian Cancer Patients. Huang R, Lin Y, Flowers L, Lisoukov H, Wang Q, Shi Q, Horowitz IR, Parthasarathy S, Huang RP. Cancer Genomics Proteomics. 2004;1(1):23-32.

2003

• Cytokine regulation by peroxisome proliferator-activated receptor gamma in human endometrial cells. Wanichkul T, Han S, Huang RP, Sidell N. Fertil Steril. 2003;79 Suppl 1:763-9.
• Detection of multiple cytokines by protein arrays from cell lysate and tissue lysate. Lin Y, Huang R, Cao X, Wang SM, Shi Q, Huang RP. Clin Chem Lab Med. 2003;41(2):139-45.
• Profiling of cytokine expression by biotin-labeled-based protein arrays. Lin Y, Huang R, Chen LP, Lisoukov H, Lu ZH, Li S, Wang CC, Huang RP. Proteomics. 2003;3(9):1750-7.

2002

• Analysis of gene expression in Egr-1 transfected human fibrosarcoma cells. Liu YG, Gano J, Huang R, Lin Y, Wang SM, Huang RP. J Surg Oncol. 2002;80(4):190-6.
• Array-based multiplexed screening and quantitation of human cytokines and chemokines. Wang CC, Huang RP, Sommer M, Lisoukov H, Huang R, Lin Y, Miller T, Burke J. J Proteome Res. 2002;1(4):337-43.
• Connexin 43 suppresses human glioblastoma cell growth by down-regulation of monocyte chemotactic protein 1, as discovered using protein array technology. Huang R, Lin Y, Wang CC, Gano J, Lin B, Shi Q, Boynton A, Burke J, Huang RP. Cancer Res. 2002;62(10):2806-12.
• Profiling of human cytokines in healthy individuals with vitamin E supplementation by antibody array. Lin Y, Huang R, Santanam N, Liu YG, Parthasarathy S, Huang RP. Cancer Lett. 2002;187(1-2):17-24.

2001

• Connexin 43 (cx43) enhances chemotherapy-induced apoptosis in human glioblastoma cells. Huang RP, Hossain MZ, Huang R, Gano J, Fan Y, Boynton AL. Int J Cancer. 2001;92(1):130-8.
• Detection of multiple proteins in an antibody-based protein microarray system. Huang RP. J Immunol Methods. 2001;255(1-2):1-13.
• Enhanced apoptosis under low serum conditions in human glioblastoma cells by connexin 43 (Cx43). Huang R, Liu YG, Lin Y, Fan Y, Boynton A, Yang D, Huang RP. Mol Carcinog. 2001;32(3):128-38.
• Simultaneous detection of multiple cytokines from conditioned media and patient's sera by an antibody-based protein array system. Huang RP, Huang R, Fan Y, Lin Y. Anal Biochem. 2001;294(1):55-62.
• Simultaneous detection of multiple proteins with an array-based enzyme-linked immunosorbent assay (ELISA) and enhanced chemiluminescence (ECL). Huang RP. Clin Chem Lab Med. 2001;39(3):209-14.

Review Articles (24)

2026

• Advances in protein microarray-based proteomics for gastric cancer applications in biomarker discovery, molecular subtyping, and precision oncology. Wang P, Xiao YL, Luo S, Dong H, Huang RP. Front Oncol. 2026;16:1907652.
• Mechanisms of therapy resistance in the tumor microenvironment: Insights from antibody array-based cytokine profiling. Wickramasekara R, Jones V, Zhao Y, Luo S, Sun G, Huang RP. Drug Resist Updat. 2026;86:101378.
• Proteomics in Antitumor Drug Target Discovery, Toxicity, Resistance, and Precision Oncology. Zhong Q, Wu X, Wiechec E, Yang Y, Liao M, Zhou H, Gao S, Li Y, Luo S, Xu L. Drug Des Devel Ther. 2026;20:629658.
• Proteomics of aging: biomarkers for physiological systems and diseases. Luo Y, Xiao YL, Chen JH, Wang H, Luo S, Dong H, Huang RP. Front Aging. 2026;7:1895104.

2025

• Advances in preparation techniques and antimicrobial properties of Aloe vera extract. Martin SS, Zhang L, Wang Y, Zhou Z, Ma R, Mo H, Tan Z, Shi H, Wang T. Fitoterapia. 2025;185:106696.
• Unraveling tumor cell‑tumor microenvironment crosstalk through antibody array technologies (Review). Wang Y, Luo S, Dong H, Huang RP. Int J Oncol. 2025;67(4).

2024

• Constructions, Purifications and Applications of DNA-Antibody Conjugates: A Review. Wang T, Wang X, Luo S, Zhang P, Li N, Chen C, Li J, Shi H, Dong H, Huang RP. ACS Omega. 2024;9(49):47951-47963.

2022

• Emerging trends in the nanomedicine applications of functionalized magnetic nanoparticles as novel therapies for acute and chronic diseases. Dash S, Das T, Patel P, Panda PK, Suar M, Verma SK. J Nanobiotechnology. 2022;20(1):393.

2021

• Non-coding RNAs and their bioengineering applications for neurological diseases. Das T, Das TK, Khodarkovskaya A, Dash S. Bioengineered. 2021;12(2):11675-11698.

2019

• Quantitative proteomic analyses in blood: A window to human health and disease. Whittaker K, Burgess R, Jones V, Yang Y, Zhou W, Luo S, Wilson J, Huang RP. J Leukoc Biol. 2019;106(3):759-775.

2018

• Integration of Antibody Array Technology into Drug Discovery and Development. Huang W, Whittaker K, Zhang H, Wu J, Zhu SW, Huang RP. Assay Drug Dev Technol. 2018;16(2):74-95.
• New Insights into the Tumor Microenvironment Utilizing Protein Array Technology. Huang W, Luo S, Burgess R, Yi YH, Huang GF, Huang RP. Int J Mol Sci. 2018;19(2).

2016

• Application of multiplex immunoassay technology to investigations of ocular disease. Jones VS, Wu J, Zhu SW, Huang RP. Expert Rev Mol Med. 2016;18:e15.
• Cytokines in cancer drug resistance: Cues to new therapeutic strategies. Jones VS, Huang RY, Chen LP, Chen ZS, Fu L, Huang RP. Biochim Biophys Acta. 2016;1865(2):255-65.

2015

• Deciphering Asthma Biomarkers with Protein Profiling Technology. Kuang Z, Wilson JJ, Luo S, Zhu SW, Huang RP. Int J Inflam. 2015;2015:630637.

2014

• Tumor-induced perturbations of cytokines and immune cell networks. Burkholder B, Huang RY, Burgess R, Luo S, Jones VS, Zhang W, ..., Lv ZQ, Gao CY, Wang BL, Zhang YM, ..., Huang RP. Biochim Biophys Acta. 2014;1845(2):182-201.

2013

• Peeking into the black box: how cytokine antibody arrays shed light on molecular mechanisms of breast cancer development and its treatment. Lin Y, Luo S, Shao N, Wang S, Duan C, Burkholder B, Xi Y, Huang RP. Curr Proteomics. 2013.

2012

• Cytokine antibody arrays in biomarker discovery and validation. Huang RP, Burkholder B, Jones V, Jiang WD, Mao YQ, Chen QL, Shi Z. Curr Proteomics. 2012;9.
• Overcoming the deficiency of singular detection by antibody arrays in cancer biomarker discovery. Jiang WD, Huang RP. J Mol Biomark Diagn. 2012;3(4).

2007

• An array of possibilities in cancer research using cytokine antibody arrays. Huang RP. Expert Rev Proteomics. 2007;4(2):299-308.

2005

• The promise of cytokine antibody arrays in the drug discovery process. Huang RP, Yang W, Yang D, Flowers L, Horowitz IR, Cao X, Huang R. Expert Opin Ther Targets. 2005;9(3):601-15.

2004

• Multiplexed sandwich immunoassays (ELISA-based protein arrays). Huang RP, Lin Y, Chen L, Yang W, Huang R. Curr Proteomics. 2004;1:199-210.

2003

• Cytokine antibody arrays: a promising tool to identify molecular targets for drug discovery. Huang RP. Comb Chem High Throughput Screen. 2003;6(8):769-75.
• Protein arrays, an excellent tool in biomedical research. Huang RP. Front Biosci. 2003;8:d559-76.

Methods and Book Chapters (16)

2021

• Analyzing Signaling Pathways Using Antibody Arrays. Tang H, Duan C, Kuang Z, Huang RP. Methods Mol Biol. 2021;2237:225-236.
• Cytometry Multiplex Bead Antibody Array. Zhang B, Xiao G, Mao YQ, Lv Z, Huang RP. Methods Mol Biol. 2021;2237:83-92.
• Data Analysis for Antibody Arrays. Zhang H, Mao YQ, Petritis B, Huang RP. Methods Mol Biol. 2021;2237:263-276.
• Dried Blood-Based Protein Profiling Using Antibody Arrays. Whittaker KC, Mao YQ, Zhu S, Lv Z, Huang RP. Methods Mol Biol. 2021;2237:199-206.
• Enhanced Protein Profiling Arrays for High-Throughput Quantitative Measurement of Cytokine Expression. Huang R, Gao CH, Wu W, Huang RP. Methods Mol Biol. 2021;2237:123-128.
• Global Detection of Proteins by Label-Based Antibody Array. Kuang Z, Chen LP, Huang R, Huang RP. Methods Mol Biol. 2021;2237:45-53.
• Methods for Membranes and Other Absorbent Surfaces. Wilson JJ, Xi Y, Huang R, Huang RP. Methods Mol Biol. 2021;2237:129-139.
• One-Step Antibody Arrays. Mao YQ, Li LH. Methods Mol Biol. 2021;2237:39-44.
• Sandwich-Based Antibody Arrays for Protein Detection. Mao YQ, Petritis B, Huang RP. Methods Mol Biol. 2021;2237:1-10.
• Statistical Analysis Options for Antibody Array Data. Lu J. Methods Mol Biol. 2021;2237:277-314.
• Surface Modification of Glass Slides with Aminosilanes for Microarray Use. Stuart C. Methods Mol Biol. 2021;2237:191-198.
• Using Antibody Arrays for Biomarker Discovery. Luo S, Ling Y, Chen LP, Huang RP. Methods Mol Biol. 2021;2237:237-245.

2016

• Cancer Stem Cell Biomarker Discovery Using Antibody Array Technology. Burgess R, Huang RP. Adv Clin Chem. 2016;73:109-25.

2015

• Antibody arrays in biomarker discovery. Wilson JJ, Burgess R, Mao YQ, Luo S, Tang H, Jones VS, Weisheng B, Huang RY, Chen X, Huang RP. Adv Clin Chem. 2015;69:255-324.

2005

• Enhanced protein profiling arrays for quantitative measurement of protein expression in multiple samples. Huang R, Shi Q, Yang W, Huang RP. Methods Mol Med. 2005;114:209-21.

2004

• Cytokine protein arrays. Huang RP. Methods Mol Biol. 2004;264:215-31.