RayPlex^® Human SIRP alpha Bead Assay

Singleplex cytometric bead assay designed to quantitatively measure a single protein target using flow cytometry. Optimized for serum, plasma, and cell culture supernatant.

$240.00

Singleplex cytometric bead assay designed to quantitatively measure a single protein target using flow cytometry. Optimized for serum, plasma, and cell culture supernatant.

Product Description

Specifications

Size	48 Tests, 96 Tests, 192 Tests, 480 Tests
Estimated Lead Time	2-3 weeks
Species	Human
Compatible Sample Types	Plasma, Cell Culture Supernatants, Serum
Accession Number	P78324
Gene Id	140885
Gene Symbols	SIRPA, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP
Protein Name / Synonyms	Tyrosine-protein phosphatase non-receptor type substrate 1 (SHP substrate 1, SHPS-1); Brain Ig-like molecule with tyrosine-based activation motifs (Bit); CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MyD-1 antigen; Signal-regulatory protein alpha-1 (Sirp-alpha-1); Signal-regulatory protein alpha-2 (Sirp-alpha-2); Signal-regulatory protein alpha-3 (Sirp-alpha-3); p84
Quantitative/Semi-Quantitative	Quantitative
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.
Note	This bead assay uses an antibody pair and standard previously validated for RayBiotech's multiplex bead array products but not yet fully validated for the singleplex format. Therefore, initial orders require a 2-3 week development and validation phase, which begins upon receipt of an order. Once the assay has passed RayBiotech's quality control testing, subsequent orders will have a typical lead time of 1-2 business days.

Product Features

4 hour processing time
Compatible with most flow cytometers
High specificity and system reproducibility
Well-suited for high throughput assays

Kit Components

Capture Beads
Assay Diluent
Serum/Plasma Diluent
Wash Buffer
Lyophilized Protein Standard
Biotinylated Detection Antibody
PE Conjugate
Flow Cytometer Setup Beads
Filler Beads
V-bottom 96-well Microplate

Additional Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue (or green) laser capable of measuring phycoerythrin (PE)
Aluminum foil or 96-well Plate Aluminum Sealers
Deionized (DI) water
1.5 mL polypropylene microcentrifuge tubes or similar
Microcentrifuge
Table centrifuge with plate holder
Recommended: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Assay Procedure Summary

Prepare Reagents, Standards, and Samples
Add Capture Beads to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody
Incubate with PE Conjugate
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (FB-PRELIM.pdf)

SDS (FB-PRELIM.pdf)

Excel (RayPlex-Analysis-Tool.xlsm)

Product Features

4 hour processing time
Compatible with most flow cytometers
High specificity and system reproducibility
Well-suited for high throughput assays

Kit Components

Capture Beads
Assay Diluent
Serum/Plasma Diluent
Wash Buffer
Lyophilized Protein Standard
Biotinylated Detection Antibody
PE Conjugate
Flow Cytometer Setup Beads
Filler Beads
V-bottom 96-well Microplate

Additional Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue (or green) laser capable of measuring phycoerythrin (PE)
Aluminum foil or 96-well Plate Aluminum Sealers
Deionized (DI) water
1.5 mL polypropylene microcentrifuge tubes or similar
Microcentrifuge
Table centrifuge with plate holder
Recommended: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Assay Procedure Summary

Prepare Reagents, Standards, and Samples
Add Capture Beads to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody
Incubate with PE Conjugate
Analyze with a compatible flow cytometer
Perform data analysis

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