Recombinant Chryseobacterium miricola PNGase F, with a C-terminal His-tag. Derived from the transfected human HEK293 cells.
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Bacteria |
| Accession Number | P21163 |
| Gene Symbols | ngl, png |
| Protein Name / Synonyms | Peptide-N(4)-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F (PNGase F); Glycopeptide N-glycosidase; N-glycanase |
| Expressed Region | Ala41-Asn354 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-Avi tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining. See gel image below. |
| Molecular Weight (kDa) | The predicted molecular weight is about 35 kDa. The actual SDS-PAGE running showed two major protein bands (35, 40 kDa). |
| Format | Liquid |
| Formulation | Filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific. See vial label, determined by Pierce BCA protein assay reagent. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
